Anderson H R, Stitt A W, Gardiner T A, Archer D B
Department of Ophthalmology, Queen's University of Belfast, Northern Ireland.
Anal Quant Cytol Histol. 1994 Aug;16(4):253-60.
The modern stereologic method of vertical sections was applied to the retina as a means of generating unbiased estimates of three-dimensional structure. The method is illustrated with real data on the volume and surface area of the capillary basement membrane from the central retina of the rat. Novel methods of estimating the volume of retina sampled and of creating accurate vertical sections are described. The advantages of using stereologic methods to generate quantitative information on the three-dimensional structure of the retina are discussed and compared to those of previous quantitative methods that provide data on two-dimensional structure only.
现代垂直切片立体学方法应用于视网膜，作为生成三维结构无偏估计的一种手段。该方法通过大鼠中央视网膜毛细血管基底膜体积和表面积的实际数据进行说明。描述了估计采样视网膜体积和创建精确垂直切片的新方法。讨论了使用立体学方法生成视网膜三维结构定量信息的优势，并与仅提供二维结构数据的先前定量方法的优势进行了比较。
