非霍奇金淋巴瘤新鲜样本与石蜡包埋样本的DNA流式细胞术比较。
Comparison of DNA flow cytometry from fresh and paraffin embedded samples of non-Hodgkin's lymphoma.
作者信息
Camplejohn R S, Macartney J C
出版信息
J Clin Pathol. 1985 Oct;38(10):1096-9. doi: 10.1136/jcp.38.10.1096.
Abstract
Cell suspensions were prepared from fresh and paraffin embedded samples of lymph nodes from nine patients with non-Hodgkin's lymphoma. DNA flow cytometry was performed on these samples and the results from fresh tissue compared with those from paraffin embedded material. Results were compared in terms of DNA index (as a measure of aneuploidy) and S phase fraction (as an indication of proliferative activity). Good agreement was found between the results from the samples prepared by the two methods. The quality of DNA flow cytometry from paraffin embedded material was comparable with or better than that from fresh samples.
摘要
从9例非霍奇金淋巴瘤患者的新鲜和石蜡包埋淋巴结样本中制备细胞悬液。对这些样本进行DNA流式细胞术检测，并将新鲜组织的结果与石蜡包埋材料的结果进行比较。根据DNA指数（作为非整倍体的指标）和S期分数（作为增殖活性的指标）对结果进行比较。发现两种方法制备的样本结果之间具有良好的一致性。石蜡包埋材料的DNA流式细胞术质量与新鲜样本相当或更好。
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