Kuesel A C, Donnelly S M, Halliday W, Sutherland G R, Smith I C
Institute for Biodiagnostics, National Research Council, Winnipeg, MB, Canada.
NMR Biomed. 1994 Jun;7(4):172-80. doi: 10.1002/nbm.1940070404.
To examine metabolic heterogeneity in primary brain tumour, multiple biopsies (one to five per tumour) from four benign meningiomas, five acoustic schwannomas, eleven glial tumours and five medulloblastomas were each subdivided into one to five samples (total = 194) and investigated using ex vivo 360 MHz 1H MR spectroscopy with histopathological correlation. Low amounts of mobile lipids were detected in meningothelial meningiomas and medulloblastomas, high amounts in some or all samples of all other tumours. The differences in non-lipid metabolites between different tumour types were less marked, with the exception of the medulloblastomas, which had high amounts of N-acetyl aspartate and cholines. The amount of mobile lipid correlated with Antoni type B areas in schwannomas and necrosis in a high grade giant cell tumour. The amount of lipid varied significantly from sample to sample in a grade 2 astrocytoma and grade 4 astrocytoma samples with 0-5% necrosis but did not correlate with the site in the tumour or histopathological characteristics. The metabolic heterogeneity occurred on a spatial scale at least as small as the size of the MR samples, i.e., < or = 60 mg. These results suggest that the detection of metabolic heterogeneity and mobile lipids may have importance for the design of clinical studies.
为研究原发性脑肿瘤中的代谢异质性，对4例良性脑膜瘤、5例听神经瘤、11例胶质瘤和5例髓母细胞瘤进行多次活检（每个肿瘤1至5次），每次活检样本再细分为1至5个样本（共194个），并采用离体360 MHz氢磁共振波谱进行研究，同时进行组织病理学对照。在脑膜内皮型脑膜瘤和髓母细胞瘤中检测到少量可移动脂质，在所有其他肿瘤的部分或全部样本中检测到大量可移动脂质。不同肿瘤类型之间非脂质代谢物的差异不太明显，但髓母细胞瘤除外，其N-乙酰天门冬氨酸和胆碱含量较高。可移动脂质的含量与神经鞘瘤中的Antoni B型区域以及高级别巨细胞瘤中的坏死相关。在坏死率为0 - 5%的2级星形细胞瘤和4级星形细胞瘤样本中，脂质含量在不同样本间差异显著，但与肿瘤部位或组织病理学特征无关。代谢异质性至少在与磁共振样本大小相同的空间尺度上出现，即≤60毫克。这些结果表明，代谢异质性和可移动脂质的检测可能对临床研究设计具有重要意义。
