Kuesel A C, Sutherland G R, Halliday W, Smith I C
Institute for Biodiagnostics, National Research Council, Winnipeg, MB, Canada.
NMR Biomed. 1994 May;7(3):149-55. doi: 10.1002/nbm.1940070308.
Sixty-four samples from six grade 4 astrocytomas were investigated ex vivo by 1H MRS at 360 MHz and subsequently by histopathology to obtain percentages of viable and necrotic tumour and grey and white matter. MR-visible lipids were detected in 87% of tumour samples. Necrotic foci were < 3 x 3 x 6 mm3. The means of the intensities/unit weight tissue of the lipid resonances at 5.33, 2.80, 1.29 and 0.89 ppm were significantly higher (p < 0.05) for three sets of comparisons: samples with 85-100% vs 50-75%; with 50-75% vs 10-40% and with 10-40% vs 0-5% necrosis. For the lipid resonance at 2.04 ppm the difference in the means was significant only for samples with 50-75% compared to those with 85-100% necrosis, because for samples with < 50% necrosis resonances from glutamine and possibly small amounts of glutamate, gamma-aminobutyrate and N-acetylaspartate anions contribute significantly to the spectral area at 2.0 ppm. We conclude that necrotic foci below MRI resolution yield the resonances at 1.3 and 0.9 ppm, and contribute to the intense resonance at 2.0 ppm observed in in vivo 1H spectra of some high grade astrocytomas.
对6例4级星形细胞瘤的64个样本进行了360 MHz的离体1H磁共振波谱（1H MRS）研究，随后进行组织病理学检查，以获取存活肿瘤、坏死肿瘤以及灰质和白质的百分比。87%的肿瘤样本中检测到磁共振可见脂质。坏死灶小于3×3×6立方毫米。在5.33、2.80、1.29和0.89 ppm处脂质共振的强度/单位重量组织的平均值在三组比较中显著更高（p<0.05）：坏死率为85%-100%的样本与坏死率为50%-75%的样本；坏死率为50%-75%的样本与坏死率为10%-40%的样本；坏死率为10%-40%的样本与坏死率为0%-5%的样本。对于2.04 ppm处的脂质共振，只有坏死率为50%-75%的样本与坏死率为85%-100%的样本之间的平均值差异显著，因为对于坏死率小于50%的样本，谷氨酰胺以及可能少量的谷氨酸、γ-氨基丁酸和N-乙酰天门冬氨酸阴离子的共振对2.0 ppm处的光谱面积有显著贡献。我们得出结论，低于MRI分辨率的坏死灶产生1.3和0.9 ppm处的共振，并对一些高级别星形细胞瘤的体内1H谱中观察到的2.0 ppm处的强共振有贡献。
