Gribbestad I S, Petersen S B, Fjøsne H E, Kvinnsland S, Krane J
MR Center, Natural Science Section, SINTEF UNIMED, University of Trondheim, Norway.
NMR Biomed. 1994 Jun;7(4):181-94. doi: 10.1002/nbm.1940070405.
High-resolution 1H NMR spectra were obtained from perchloric acid extracts of breast tumor specimens and adjacent non-involved tissue. Two-dimensional shift-correlated and homonuclear J-resolved spectroscopy were used to identify coupled resonances in the spectra. Chemical shifts, multiplicities and spin-spin coupling constants of several non-resolved resonances in the one-dimensional spectra could be determined by the two-dimensional methods. Several differences in the metabolite content of the two types of extracts were established. The spectra of extracts from non-involved tissue were dominated by signals from glucose and other carbohydrates, while most of the tumors had very low or no detectable levels of glucose. High concentrations of lactate, taurine and succinate, an increase of the phosphocholine level, and a very low phosphocreatine level were characteristic findings in the 1H spectra of tumor extracts. The variation in the level of myo-inositol follows the variation in glucose for the two types of tissue. Scyllo-inositol was for the first time observed in the NMR spectra from breast tissue. Uridine 5'-diphospho-N-acetylglucosamine and uridine 5'-diphospho-N-acetylgalactosamine have been identified and there is an increased level of these two hexoses in the tumor tissue. These results provide insight into breast tumor metabolism, by simultaneously detecting a large number of metabolites and demonstrate the potential for using 1H NMR spectroscopy for studying different metabolic pathways in breast tumors. At the same time they provide useful information for interpretation of in vivo 1H NMR spectra of breast tumors.
从乳腺肿瘤标本及相邻未受累组织的高氯酸提取物中获得了高分辨率的1H NMR光谱。使用二维位移相关光谱和同核J分辨光谱来识别光谱中的耦合共振。一维光谱中几个未分辨共振的化学位移、多重性和自旋-自旋耦合常数可以通过二维方法确定。确定了两种提取物代谢物含量的几个差异。未受累组织提取物的光谱主要由葡萄糖和其他碳水化合物的信号主导，而大多数肿瘤的葡萄糖水平非常低或无法检测到。肿瘤提取物的1H光谱中的特征性发现包括高浓度的乳酸、牛磺酸和琥珀酸、磷酸胆碱水平升高以及磷酸肌酸水平极低。两种组织中肌醇水平的变化与葡萄糖的变化一致。首次在乳腺组织的NMR光谱中观察到scyllo-肌醇。已鉴定出尿苷5'-二磷酸-N-乙酰葡糖胺和尿苷5'-二磷酸-N-乙酰半乳糖胺，并且肿瘤组织中这两种己糖的水平有所增加。这些结果通过同时检测大量代谢物，深入了解了乳腺肿瘤代谢，并证明了使用1H NMR光谱研究乳腺肿瘤不同代谢途径的潜力。同时，它们为解释乳腺肿瘤的体内1H NMR光谱提供了有用的信息。
