Monfrecola G, Procaccini E M, Schettino A, Calabrò G, Manco A, Santoianni P
Clinica Dermatologica, Facoltà di Medicina, Universita degli Studi Federico II, Napoli, Italy.
Photodermatol Photoimmunol Photomed. 1994 Jun;10(3):102-5.
Ultraviolet B and psoralen plus UVA treatment induce antigenic and enzymatic changes in Langerhans cells (LC). The aim of this study was to investigate the visible (VIS) effect on mice LC surface markers. As visible source, a slide projector equipped with a 150-W tungsten lamp, emitting between 400 and 740 nm (maximum at 580 nm) was used. Mice (BALB/c and C3H) were divided into groups, each irradiated with visible single fixed doses (ranging from 10 to 1000 J/cm2). The mice backs were shaved before irradiation. Skin biopsies obtained immediately after irradiation were processed for immunofluorescence and electron microscopy. Immunofluorescent studies showed: 1) a complete depletion of LC membrane markers at a dose of 700 J/cm2; 2) no effect at visible doses ranging between 0 and 75 J/cm2; 3) a dose-dependent effect with doses between 100 and 700 J/cm2. Electron microscopy revealed no cellular damage of LC at the visible doses administered.
紫外线B以及补骨脂素加紫外线A疗法可诱导朗格汉斯细胞（LC）发生抗原性和酶促变化。本研究旨在探究可见光（VIS）对小鼠LC表面标志物的影响。作为可见光源，使用了一台配备150瓦钨灯的幻灯机，其发射波长在400至740纳米之间（峰值为580纳米）。将小鼠（BALB/c和C3H）分成若干组，每组用单一固定剂量的可见光照射（剂量范围为10至1000焦耳/平方厘米）。照射前将小鼠背部毛发剃除。照射后立即获取皮肤活检样本，用于免疫荧光和电子显微镜检查。免疫荧光研究显示：1）剂量为700焦耳/平方厘米时，LC膜标志物完全耗尽；2）可见光剂量在0至75焦耳/平方厘米之间时无影响；3）剂量在100至700焦耳/平方厘米之间时呈剂量依赖性效应。电子显微镜检查显示，所给予的可见光剂量对LC无细胞损伤。
