Establishment of the two glioma cell lines: YH and AM.

We have established two human glioma cell lines, which were designated as YH cells and AM cells. The two cell lines have maintained morphological appearance as seen in the primary culture and immunohistochemically expressed glial fibrillary acidic protein (GFAP) and S-100 protein. Population doubling time for YH cells and AM cells indicated 30 hours and 25 hours, respectively, in an exponential phase of culture.