胶质瘤细胞在培养过程中的表型改变。
Phenotypic alteration of glioma cells during culture.
作者信息
Imai F, Marunouchi T, Mizoguchi Y, Ogasawara M, Kanno T
机构信息
Department of Neurosurgery, School of Medicine, Fujita Health University.
出版信息
Noshuyo Byori. 1993;10(1):19-25.
Tissue specimens and culture cells from three human gliomas (two astrocytomas and one glioblastoma) were immunohistochemically investigated, using GFAP, S-100P, vimentin, FN and TNF antibodies. Primary culture consisted of two cell types, flat cells and fibrous cells. Phenotypic alternation was observed during successive subculture. Differences between fibrous cells in astrocytoma and those in glioblastoma were remarkable, while flat cells in astrocytoma and glioblastoma examined in this study, were similar.
使用GFAP、S-100P、波形蛋白、纤连蛋白和肿瘤坏死因子抗体，对来自三例人类胶质瘤（两例星形细胞瘤和一例胶质母细胞瘤）的组织标本和培养细胞进行了免疫组织化学研究。原代培养由两种细胞类型组成，即扁平细胞和纤维细胞。在连续传代培养过程中观察到了表型改变。星形细胞瘤中的纤维细胞与胶质母细胞瘤中的纤维细胞之间的差异显著，而本研究中检测的星形细胞瘤和胶质母细胞瘤中的扁平细胞则相似。
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