Dobak J, Grzybowski J, Liu F T, Landon B, Dobke M
Division of Plastic Surgery, University of California San Diego 92103-8890.
J Dermatol Sci. 1994 Aug;8(1):18-24. doi: 10.1016/0923-1811(94)90316-6.
The effect of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on proliferation and collagen type I and type III production in cultured human fibroblasts was examined. Previous studies have identified receptors for the vitamin in human dermal fibroblasts, and have suggested the skin to be a target tissue. While many studies examining keratinocyte modulation by 1,25-(OH)2D3 have been undertaken, very few have been performed on dermal fibroblasts. Neonatal foreskin fibroblast cultures were examined for cell number, extracellular collagen accumulation, and collagen mRNA levels, after 5 days exposure to 1,25-(OH)2D3 at a concentration of 10(-7) M. The vitamin significantly suppressed (P < 0.01) the proliferation of fibroblasts cultured in the presence of serum. Day 5 cell culture supernatants showed a significant per cell increase in collagen type I (P < 0.05) and type III (P < 0.01) as measured by ELISA. Type I collagen production in exposed cells was 11.64 + 0.531 microgram/10(6) cell vs. 9.53 + 0.500 microgram/10(6) cells in unexposed cells. Type III collagen production was 0.601 + 0.012 microgram/10(6) cell in exposed cells and 0.247 + 0.008 microgram/10(6) cells in unexposed cells. mRNA levels were increased after a 4-day exposure to 10(-7) M 1,25-(OH)2D3 for both type I (2.5-5-fold) and type III (5.5-7.76-fold) collagen. These results suggest a novel effect of increased collagen production by dermal fibroblasts upon exposure to 1,25-(OH)2D3 that is independent of proliferation.
研究了1,25 - 二羟基维生素D3（1,25-(OH)2D3）对培养的人成纤维细胞增殖以及I型和III型胶原蛋白生成的影响。先前的研究已在人真皮成纤维细胞中鉴定出该维生素的受体，并表明皮肤是一个靶组织。虽然已经进行了许多关于1,25-(OH)2D3对角质形成细胞调节作用的研究，但对真皮成纤维细胞的研究却很少。将新生儿包皮成纤维细胞培养物暴露于浓度为10(-7) M的1,25-(OH)2D3 5天后，检测细胞数量、细胞外胶原蛋白积累和胶原蛋白mRNA水平。该维生素显著抑制（P < 0.01）在有血清存在的情况下培养的成纤维细胞的增殖。通过ELISA检测，第5天的细胞培养上清液显示，每细胞的I型胶原蛋白（P < 0.05）和III型胶原蛋白（P < 0.01）显著增加。暴露细胞中I型胶原蛋白的产量为11.64 + 0.531微克/10(6)细胞，未暴露细胞中为9.53 + 0.500微克/10(6)细胞。III型胶原蛋白产量在暴露细胞中为0.601 + 0.012微克/10(6)细胞，未暴露细胞中为0.247 + 0.008微克/10(6)细胞。在暴露于10(-7) M 1,25-(OH)2D3 4天后，I型（2.5 - 5倍）和III型（5.5 - 7.76倍）胶原蛋白的mRNA水平均升高。这些结果表明，真皮成纤维细胞暴露于1,25-(OH)2D3后胶原蛋白生成增加是一种新的作用，且与增殖无关。
