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编码大鼠PKR（双链RNA依赖的真核起始因子2激酶）的cDNA的克隆与鉴定

Cloning and characterization of a cDNA encoding rat PKR, the double-stranded RNA-dependent eukaryotic initiation factor-2 kinase.

作者信息

Mellor H, Flowers K M, Kimball S R, Jefferson L S

机构信息

Department of Cellular and Molecular Physiology, College of Medicine, Pennsylvania State University, Hershey 17033.

出版信息

Biochim Biophys Acta. 1994 Nov 22;1219(3):693-6. doi: 10.1016/0167-4781(94)90229-1.

DOI:10.1016/0167-4781(94)90229-1
PMID:7948027
Abstract

We have isolated and sequenced a full-length cDNA encoding the double-stranded RNA-dependent protein kinase PKR from rat. The derived amino acid sequences of the protein kinase and RNA-binding domains are highly conserved between the rat, human and mouse enzymes. In addition, sequence elements in the 5'- and 3'-untranslated regions are also conserved between species.

摘要

我们从大鼠中分离并测序了编码双链RNA依赖性蛋白激酶PKR的全长cDNA。该蛋白激酶和RNA结合结构域的推导氨基酸序列在大鼠、人类和小鼠的酶之间高度保守。此外，5'和3'非翻译区的序列元件在不同物种之间也保守。

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