Ródenas V, Parra A, Garcia-Villanova J, Gómez M D
Servicio de Análisis Clínicos, Hospital Virgen del Castillo, Murcia, Spain.
J Pharm Biomed Anal. 1995 Aug;13(9):1095-9. doi: 10.1016/0731-7085(95)01507-h.
A simple spectrophotometric assay for the determination of cefepime and L-arginine in injections is described. Since zero-order spectra showed considerable overlap, second-derivative spectrophotometry was used to enhance the spectral details. A linear relationship between second-derivative amplitude and concentration of each compound was found. Beer's law was obeyed up to 50 and 22 micrograms ml-1 of cefepime and arginine, respectively, in the second-derivative mode. Detection limits were 0.31 and 0.58 micrograms ml-1 for cefepime and arginine, respectively. The method, which is rapid, simple and does not require any separation step, has been successfully applied to the assay of commercial injections containing cefepime and arginine.
描述了一种用于测定注射剂中头孢吡肟和L-精氨酸的简单分光光度法。由于零阶光谱显示出相当大的重叠，因此使用二阶导数分光光度法来增强光谱细节。发现每种化合物的二阶导数幅度与浓度之间存在线性关系。在二阶导数模式下，头孢吡肟和精氨酸的浓度分别高达50和22微克/毫升时均符合比尔定律。头孢吡肟和精氨酸的检测限分别为0.31和0.58微克/毫升。该方法快速、简单，不需要任何分离步骤，已成功应用于含头孢吡肟和精氨酸的市售注射剂的测定。
