Bryan P D, Stewart J T
Department of Medicinal Chemistry, College of Pharmacy, University of Georgia, Athens 30602-2352.
J Pharm Biomed Anal. 1994 May;12(5):675-92. doi: 10.1016/0731-7085(93)e0013-d.
The LC analysis of selected tetracyclines from dosage forms and bulk drug substance using polymeric columns has been studied. Mobile phases containing acetonitrile-0.02 M sodium perchlorate (pH 2.0) were used. The tetracyclines were detected by their absorbance at 280 nm. The columns included: a polystyrene-divinylbenzene (PS-DVB) column and a polymethacrylate column with octadecyl ligands (PM-C18). Performance of the two columns was compared and applicability of the described methods for compendial use has been evaluated. The tetracyclines investigated include: minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline, methacycline, doxycycline and meclocycline.
已对使用聚合物柱对剂型和原料药中选定四环素进行的液相色谱分析进行了研究。使用了含有乙腈 - 0.02 M 高氯酸钠（pH 2.0）的流动相。通过在 280 nm 处的吸光度检测四环素。这些色谱柱包括：聚苯乙烯 - 二乙烯基苯（PS - DVB）柱和带有十八烷基配体的聚甲基丙烯酸酯柱（PM - C18）。比较了这两种色谱柱的性能，并评估了所述方法在药典用途中的适用性。所研究的四环素包括：米诺环素、土霉素、四环素、地美环素、金霉素、甲烯土霉素、强力霉素和甲氯环素。
