Bryan P D, Stewart J T
College of Pharmacy, Department of Medicinal Chemistry, University of Georgia, Athens 30602-2352.
J Pharm Biomed Anal. 1993 Oct;11(10):971-6. doi: 10.1016/0731-7085(93)80057-8.
The LC separation of selected tetracyclines has been studied using polymeric columns. Mobile phases containing acetonitrile-0.02 M sodium perchlorate, pH 2.0, were used. Asymmetry factor and number of theoretical plates were calculated for the tetracyclines investigated on four polymeric columns. The columns included: two polystyrene-divinylbenzene (PS-DVB) copolymeric columns, a PS-DVB column with octadecyl ligands and a polymethacrylate column with octadecyl ligands (PM-C18). The PLRP-S (PS-DVB) column and the PM-C18 column were found to be the most suitable for the analysis of the selected tetracyclines. Resolutions between pairs of selected tetracyclines were calculated and compared for the latter two columns, with the PLRP-S (PS-DVB) columns showing the best results. The PM-C18 column has the advantage of allowing the use of higher flow rates, which minimized analysis times. The tetracyclines included minocycline, oxytetracycline, tetracycline, demeclocycline, chlortetracycline, methacycline, doxycycline and meclocycline. Representative degradation products and impurities for selected tetracyclines were also included.
已使用聚合物柱研究了选定四环素的液相色谱分离。使用了含有乙腈 - 0.02 M高氯酸钠（pH 2.0）的流动相。计算了在四根聚合物柱上所研究四环素的不对称因子和理论塔板数。这些柱子包括：两根聚苯乙烯 - 二乙烯基苯（PS - DVB）共聚柱、一根带有十八烷基配体的PS - DVB柱和一根带有十八烷基配体的聚甲基丙烯酸酯柱（PM - C18）。发现PLRP - S（PS - DVB）柱和PM - C18柱最适合分析选定的四环素。计算并比较了后两根柱子上选定四环素对之间的分离度，结果显示PLRP - S（PS - DVB）柱表现最佳。PM - C18柱的优点是可以使用更高的流速，从而将分析时间减至最短。所研究的四环素包括米诺环素、土霉素、四环素、地美环素、金霉素、甲烯土霉素、多西环素和甲氯环素。还包括选定四环素的代表性降解产物和杂质。
