Killion J J, Fishbeck R, Bar-Eli M, Chernajovsky Y
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston.
Cytokine. 1994 Jul;6(4):443-9. doi: 10.1016/1043-4666(94)90069-8.
The antiproliferative effect of interferon alpha (IFN-alpha) against cultured 253J human bladder tumour cells was enhanced when IFN-alpha was encapsulated into multilamellar phospholipid liposomes (MLV). Moreover, significant cytostasis of a variant 253J subline (253J alpha R, resistant to the antiproliferative effect of IFN-alpha), could be achieved by delivery of IFN-alpha contained in liposomes. Although the two cell lines have the same number and affinity of cell receptors for IFN-alpha, the 253J alpha R cells did not down-regulate receptors as observed for the IFN-sensitive 253J cells. The IFN-response genes, 2'-5' oligosynthetase and gene 6-16 were equally induced in both cell lines following incubation of the cells with either free IFN-alpha or liposome-encapsulated IFN-alpha. Incorporation of radiolabelled IFN-alpha into cells by liposomes was independent of the status of IFN-receptors (the receptors being either occupied or down-regulated). These observations are consistent with the hypothesis that the antiproliferative effects of IFN-alpha against 253J and 253J alpha R cells may be mediated by internalization of IFN-alpha.
当干扰素α（IFN-α）被包裹在多层磷脂脂质体（MLV）中时，其对培养的253J人膀胱肿瘤细胞的抗增殖作用增强。此外，通过递送脂质体中所含的IFN-α，可实现对253J亚系变体（253JαR，对IFN-α的抗增殖作用具有抗性）的显著细胞生长抑制。尽管这两种细胞系对IFN-α的细胞受体数量和亲和力相同，但253JαR细胞不像对IFN敏感的253J细胞那样下调受体。在用游离IFN-α或脂质体包裹的IFN-α孵育细胞后，两种细胞系中的IFN反应基因2'-5'寡聚合成酶和基因6-16均被同等诱导。脂质体将放射性标记的IFN-α掺入细胞中与IFN受体的状态无关（受体要么被占据，要么被下调）。这些观察结果与以下假设一致，即IFN-α对253J和253JαR细胞的抗增殖作用可能是由IFN-α的内化介导的。
