Pellier V, Astic L
Laboratoire de Physiologie Neurosensorielle, UCB/LYON 1, Villeurbanne, France.
Neuroreport. 1994 Jul 21;5(12):1429-32. doi: 10.1097/00001756-199407000-00006.
The purpose of this study was to provide further information upon the cell death process by apoptosis occurring in the olfactory pit during the primary palate formation and the vomeronasal organ detachment. Apoptotic cells were detected by coupling ultrastructural observations and in situ end-labelling of DNA breaks (TUNEL labelling) in E12-E15 rat embryos. During the primary palate formation and the vomeronasal organ closure, a strong apoptotic cell death process was observed along the midline epithelial seam after the epithelial fusion. The topographical distribution of labelled nuclei was in agreement with the morphological distribution of dying cells. One day before the nasal swellings fused, numerous degenerating cells were also detected in the regions of prospective contact which thus appeared as regions of programmed cell death.
本研究的目的是提供更多关于在原发腭形成和犁鼻器分离过程中，嗅窝内发生的凋亡性细胞死亡过程的信息。通过对E12-E15大鼠胚胎进行超微结构观察并结合DNA断裂的原位末端标记（TUNEL标记）来检测凋亡细胞。在原发腭形成和犁鼻器闭合过程中，上皮融合后沿中线上皮缝观察到强烈的凋亡性细胞死亡过程。标记细胞核的拓扑分布与死亡细胞的形态分布一致。在鼻隆起融合前一天，在预期接触区域也检测到大量退化细胞，因此这些区域表现为程序性细胞死亡区域。
