Matute C, García-Barcina J M, Miledi R
Department of Psychobiology, University of California, Irvine 92717.
Neuroreport. 1994 Jul 21;5(12):1457-60. doi: 10.1097/00001756-199407000-00012.
Using Xenopus oocytes, we have investigated the presence of mRNA coding for neurotransmitter receptors and channels in the adult bovine fornix and optic nerve. Oocytes injected with mRNA from either area, expressed comparable levels of functional receptors to several neurotransmitters, including glutamate and angiotensin II, as well as voltage-activated Ca2+ channels. Glutamate receptors were mainly of the AMPA/kainate type and were expressed in astrocyte cultures from the regions studied, suggesting that the receptors are present at least in this type of glial cells in vivo. These results show that the white matter areas in the adult brain express receptors and channels. These molecules may play an important role in the transfer of information between axons and glial cells.
利用非洲爪蟾卵母细胞，我们研究了成年牛穹窿和视神经中编码神经递质受体及通道的mRNA的存在情况。用来自这两个区域的mRNA注射的卵母细胞，表达出与几种神经递质（包括谷氨酸和血管紧张素II）以及电压激活的Ca2+通道相当水平的功能性受体。谷氨酸受体主要是AMPA/海人酸型，并在所研究区域的星形胶质细胞培养物中表达，这表明这些受体至少在体内的这类神经胶质细胞中存在。这些结果表明，成人大脑的白质区域表达受体和通道。这些分子可能在轴突与神经胶质细胞之间的信息传递中发挥重要作用。
