Messamore E, Bogdanovich N, Schröder H, Winblad B
Karolinska Institute Alzheimer's Disease Research Centre, Department of Geriatric Medicine, Huddinge University Hospital, Sweden.
Neuroreport. 1994 Jul 21;5(12):1473-6. doi: 10.1097/00001756-199407000-00016.
AUTOPSY sections of the temporal and parahippocampal gyri were incubated with the monoclonal antibody M35 to label muscarinic acetylcholine receptors. Senile plaques were visualized either by counterstaining with Congo red or by Bielschowsky silver-stain of adjacent sections. The antibody labelled pyramidal and non-pyramidal cells in the grey matter, as well as fibrous astrocytes in the white matter and first cortical layer, of elderly control brains. In AD brains however, the grey matter was characterized by prominently labelled fibrous astrocytes and few or poorly labelled neurones. M35-immunoreactive astrocytes were often associated with senile plaques. These findings suggest that plaque-associated astrocytes may be responsive to acetylcholine or cholinergic drugs.
将颞叶和海马旁回的尸检切片与单克隆抗体M35一起孵育，以标记毒蕈碱型乙酰胆碱受体。通过用刚果红复染或对相邻切片进行 Bielschowsky 银染来观察老年斑。该抗体标记了老年对照大脑灰质中的锥体细胞和非锥体细胞，以及白质和第一皮质层中的纤维性星形胶质细胞。然而，在阿尔茨海默病（AD）大脑中，灰质的特征是纤维性星形胶质细胞标记明显，而神经元标记很少或很差。M35免疫反应性星形胶质细胞常与老年斑相关。这些发现表明，与斑块相关的星形胶质细胞可能对乙酰胆碱或胆碱能药物有反应。
