Arseniev L, Weisswange T, Neumann H J, Stangel W
Blutbank, Immunhämatologie, Transfusionsmedizin, Medizinische Hochschule, Hannover.
Infusionsther Transfusionsmed. 1994 Aug;21(4):274-7. doi: 10.1159/000222987.
Two different types of polyolefin storage containers were compared in order to estimate their ability to preserve apheresis platelet concentrates for 5 days.
Ten pairs each consisting of one patient with thrombocytopenia following chemotherapy and one healthy platelet apheresis donor were examined. The platelet concentrates stored in the LE-2 bag were collected with a Fresenius AS 104 cell separator and those stored in the PL 732 with a Fenwal CS 3000.
The separation efficiency of both cell separators was similar; the mean yields were 3.37 +/- 0.83 x 10(11) platelets in 274 +/- 26 ml for the AS 104 and 3.87 +/- 1.31 x 10(11) platelets in 318 +/- 22 ml for the CS 3000 (mean +/- SD). Storage for 5 days did not influence the platelet count significantly. The platelet loss due to filtration was 16 and 13%, respectively. The mean platelet volume obtained with both systems was reduced from a mean of 8.4 fl immediately after harvesting to 7.6 fl after storage (p < 0.0001) and to 7.3 fl after filtration (p = 0.001). The established corrected count increments (CCI) and the pre- and posttransfusion platelet counts were satisfactory and comparable for the 2 systems tested. The mean CCI of the AS 104-LE-2 system was 14.5 1 h and 7.4 x 10(9) platelets 24 h after transfusion, the mean CCI of the CS 3000-PL 732 system was 12.5 and 5.2 x 10(9) platelets, respectively.
Single-donor apheresis concentrates with a large platelet content may be stored in the new LE-2 polyolefin container for up to 5 days and used in clinical transfusion.
比较两种不同类型的聚烯烃储存容器，以评估其保存单采血小板浓缩物5天的能力。
对10对患者进行检查，每对包括一名化疗后血小板减少的患者和一名健康的血小板单采供者。用费森尤斯AS 104血细胞分离机采集储存在LE-2袋中的血小板浓缩物，用芬瓦CS 3000采集储存在PL 732中的血小板浓缩物。
两台血细胞分离机的分离效率相似；AS 104的平均产量为274±26 ml中3.37±0.83×10¹¹个血小板，CS 3000的平均产量为318±22 ml中3.87±1.31×10¹¹个血小板（平均值±标准差）。储存5天对血小板计数无显著影响。过滤导致的血小板损失分别为16%和13%。两种系统获得的平均血小板体积从采集后立即的平均8.4 fl降至储存后的7.6 fl（p<0.0001），过滤后降至7.3 fl（p = 0.001）。对于所测试的两种系统，既定的校正计数增加值（CCI）以及输血前后的血小板计数均令人满意且具有可比性。AS 104-LE-2系统输血后1小时的平均CCI为14.5，24小时为7.4×10⁹个血小板，CS 3000-PL 732系统的平均CCI分别为12.5和5.2×10⁹个血小板。
血小板含量高的单供者单采浓缩物可储存在新型LE-2聚烯烃容器中长达5天，并用于临床输血。
