Järemo P
Department of Transfusion Medicine, Orebro Medical Center Hospital, Sweden.
Infusionsther Transfusionsmed. 1994 Aug;21(4):278-80. doi: 10.1159/000222988.
The study examines Pall Pl-50 platelet filters with respect to their influence on some biochemical properties of platelet concentrates.
Ten pairs of pooled platelet preparations, each consisting of 3 platelet concentrates, were studied. On day 1, one of the preparations was filtered and the other served as an identical non-filtered control. Immediately after filtration platelet counts, the levels of desarginated activated complement factor 3 (C3a), extracellular levels of lactate dehydrogenase (LDH) and extracellular and total concentrations of platelet factor 4 (PF4) were determined in both concentrates. After 5 days sterility testing and determination of extracellular levels of PF4 and LDH were performed.
Extracellular concentrations of PF4 and C3a decreased immediately after filtration (p < 0.01). Furthermore, Pall Pl-50 filters caused cellular instability reflected by an increased release of PF4 (p < 0.01) during subsequent storage.
Platelet concentrates filtered with Pall Pl-50 would not appear suitable for storage.
本研究考察了颇尔Pl - 50血小板过滤器对血小板浓缩物某些生化特性的影响。
研究了十对混合血小板制剂，每对由3个血小板浓缩物组成。在第1天，其中一份制剂进行过滤，另一份作为相同的未过滤对照。过滤后立即测定两份浓缩物中的血小板计数、去精氨酸化活化补体因子3（C3a）水平、细胞外乳酸脱氢酶（LDH）水平以及血小板因子4（PF4）的细胞外和总浓度。5天后进行无菌测试并测定PF4和LDH的细胞外水平。
过滤后PF4和C3a的细胞外浓度立即下降（p < 0.01）。此外，颇尔Pl - 50过滤器导致细胞不稳定，表现为在随后的储存期间PF4释放增加（p < 0.01）。
用颇尔Pl - 50过滤的血小板浓缩物似乎不适合储存。
