Loutelier C, Lange C, Cassier P, Vey A, Cherton J C
Laboratoire de Chimie Organique Structurale, Université P. et M. Curie, Paris, France.
J Chromatogr B Biomed Appl. 1994 Jun 3;656(1):281-92. doi: 10.1016/0378-4347(94)00090-5.
To study the behavior of the toxins E and A destruxin in biological media, a method was developed with direct injection on to a liquid chromatographic (LC) column. Either slightly lipophilic C1 and C4 "wide-pore" packings or the column-switching approach with a guard column were used. To confirm the results of the direct LC analysis, a "classical approach" with pretreatment prior to injection on to a C8 packed column was also developed. Further, parallel fast atom bombardment MS monitoring in the negative-ion mode was carried out on the same biological samples, to obtain complementary information on the destruxin metabolism in locusts. Thus, the behaviours of E and A destruxin were examined in vivo in different organs of locusts. For E destruxin, several detoxication processes could be observed, such as hydrolysis and conjugation with glutathione.
为研究毒素E和A金龟子绿僵菌素在生物介质中的行为，开发了一种直接进样到液相色谱（LC）柱上的方法。使用了略微亲脂性的C1和C4“宽孔”填料，或采用带有保护柱的柱切换方法。为了证实直接LC分析的结果，还开发了一种在进样到C8填充柱之前进行预处理的“经典方法”。此外，对相同的生物样品进行了负离子模式下的平行快速原子轰击质谱监测，以获取有关金龟子绿僵菌素在蝗虫体内代谢的补充信息。因此，在蝗虫的不同器官中对毒素E和A金龟子绿僵菌素的行为进行了体内研究。对于毒素E金龟子绿僵菌素，可以观察到几种解毒过程，例如水解以及与谷胱甘肽的结合。
