Pettersson L, Eriksson C G
Department of Clinical Chemistry, Mälarsjukhuset, Eskilstuna, Sweden.
J Chromatogr B Biomed Appl. 1994 Jul 1;657(1):31-6. doi: 10.1016/0378-4347(94)80066-9.
A method for the determination of 7 alpha-hydroxy-4-cholesten-3-one in human serum has been developed. The method is based upon solid-phase (C18) extraction of serum after addition of 7-beta-hydroxy-4-cholesten-3-one as internal standard. The extract is analysed by reversed-phase HPLC using acetonitrile-water as a mobile phase. The effluent is monitored at 241 nm. The overall recoveries of the method range from 96% to 105%. The coefficient of variation for the within-run precision is 3.2% (n = 20, mean = 13.0 ng/ml) and for the between-run precision 3.8% (n = 32, mean = 13.3 ng/ml). The limit of detection is 3 ng at a signal-to-noise ratio of 3:1, which corresponds to 1 ng/ml using 3 ml of serum. The median value of 7 alpha-hydroxy-4-cholesten-3-one found in blood donors (n = 27) was 8.9 ng/ml (range 2-35 ng/ml).
已开发出一种测定人血清中7α-羟基-4-胆甾烯-3-酮的方法。该方法基于在加入7-β-羟基-4-胆甾烯-3-酮作为内标后对血清进行固相（C18）萃取。萃取物通过反相高效液相色谱法进行分析，以乙腈-水作为流动相。流出物在241nm处进行监测。该方法的总回收率在96%至105%之间。批内精密度的变异系数为3.2%（n = 20，平均值 = 13.0 ng/ml），批间精密度的变异系数为3.8%（n = 32，平均值 = 13.3 ng/ml）。在信噪比为3:1时检测限为3 ng，使用3 ml血清时相当于1 ng/ml。在献血者（n = 27）中发现的7α-羟基-4-胆甾烯-3-酮的中位数为8.9 ng/ml（范围为2 - 35 ng/ml）。
