Schreier P, Drawert F, Junker A, Barton H, Leupold G
Z Lebensm Unters Forsch. 1976 Nov 24;162(3):279-84. doi: 10.1007/BF01113310.
Using methionine as single nitrogen source 3-(methylthio)-1-propanol, 3-(methylthio)-propanal, 3-(methylthio)-propylacetate and 2-methylthiophan-3-one were formed in anaerobic model fermentations by Saccharomyces cerebisiae. The sulphur compounds were enriched by liquid-liquid extraction and detected by a selective flame photometric detector (FPD). The identities of the components were confirmed by mass spectrometry. It could be shown by quantitative determiniations that methionine was metabolized with 56--61% to 3-(methylthio)=1-propanol, when it was given to the medium in concentrations from 10 to 100 mg/1. 3-(methylthio)-propylacetuate, 3-(methylthio)-propanal, and 2-methylthiophan-3-one only could be detected in traces.
以蛋氨酸作为单一氮源时,酿酒酵母在厌氧模型发酵中会生成3-(甲硫基)-1-丙醇、3-(甲硫基)丙醛、3-(甲硫基)丙基乙酸酯和2-甲硫基-3-酮。这些含硫化合物通过液液萃取进行富集,并使用选择性火焰光度检测器(FPD)进行检测。各成分的身份通过质谱法得以确认。定量测定表明,当蛋氨酸以10至100 mg/1的浓度添加到培养基中时,56%至61%的蛋氨酸会代谢生成3-(甲硫基)-1-丙醇。仅能检测到痕量的3-(甲硫基)丙基乙酸酯、3-(甲硫基)丙醛和2-甲硫基-3-酮。