Torp R, Danbolt N C, Babaie E, Bjørås M, Seeberg E, Storm-Mathisen J, Ottersen O P
Department of Anatomy, University of Oslo, Norway.
Eur J Neurosci. 1994 Jun 1;6(6):936-42. doi: 10.1111/j.1460-9568.1994.tb00587.x.
The distributions of the mRNAs encoding the L-glutamate transporters GLT1 and GLAST were examined in the rat brain by in situ hybridization using 35S-labelled oligonucleotide probes. Probes directed to GLT1 produced dense labelling in the hippocampus, neocortex and neostriatum, and weak labelling in the cerebellum. In contrast, GLAST mRNA appeared to be greatly enriched in the cerebellum compared to other brain regions. While the intensity of the labelling for GLAST and GLT1 varied among different regions, their cellular distributions appeared to coincide inasmuch as both mRNAs were mainly expressed by glial cells. Labelling occurred, inter alia, in glial cells throughout the hippocampus, and in Golgi epithelial cells in the Purkinje cell layer of the cerebellum.
使用35S标记的寡核苷酸探针，通过原位杂交技术在大鼠脑中检测了编码L-谷氨酸转运体GLT1和GLAST的mRNA的分布。针对GLT1的探针在海马体、新皮层和新纹状体中产生密集标记，而在小脑中产生较弱标记。相比之下，与其他脑区相比，GLAST mRNA在小脑中似乎大量富集。虽然GLAST和GLT1标记的强度在不同区域有所不同，但它们的细胞分布似乎一致，因为两种mRNA主要由神经胶质细胞表达。标记尤其出现在整个海马体的神经胶质细胞中，以及小脑浦肯野细胞层的高尔基上皮细胞中。
