Sippel J E, Mamay H K, Weiss E, Joseph S W, Beasley W J
J Clin Microbiol. 1978 Apr;7(4):372-8. doi: 10.1128/jcm.7.4.372-378.1978.
Outer membrane protein preparations were obtained from strains of Salmonella and Neisseria meningitidis. Solubilized cell envelope (CE) fractions from S. typhi and Salmonella groups A, B, C, and E had very similar electrophoretic mobilities on polyacrylamide gel, and common antigens were demonstrated by immunodiffusion. CE appeared to be a more satisfactory antigen than the more purified preparation (T/TEI) in the enzyme-linked immunosorbent assay (ELISA) with sera from typhoid and paratyphoid patients. With either antigen, however, the presence of antibodies was demonstrated in acute- and vonvalescent-phase sera. In the case of N. meningitidis infections, the crude (STA) and the more purified antigens (T/TEI) were equally satisfactory, and a rise in antibody titer could easily be demonstrated with paired acute- and convalescent-phase sera. The ELISA appears to be a simple but highly sensitive test for the detection of antibodies by using outer membrane protein antigens.
外膜蛋白制剂取自沙门氏菌和脑膜炎奈瑟菌菌株。伤寒沙门氏菌以及A、B、C和E群沙门氏菌的溶解细胞包膜（CE）组分在聚丙烯酰胺凝胶上具有非常相似的电泳迁移率，免疫扩散显示存在共同抗原。在酶联免疫吸附测定（ELISA）中，用伤寒和副伤寒患者的血清检测时，CE似乎比纯化程度更高的制剂（T/TEI）是更令人满意的抗原。然而，无论使用哪种抗原，急性期和恢复期血清中均显示存在抗体。对于脑膜炎奈瑟菌感染，粗制抗原（STA）和纯化程度更高的抗原（T/TEI）同样令人满意，通过急性期和恢复期配对血清很容易证明抗体滴度升高。ELISA似乎是一种利用外膜蛋白抗原检测抗体的简单但高度灵敏的试验。
