Oldfield B J, Allen A M, Hards D K, McKinley M J, Schlawe I, Mendelsohn F A
Howard Florey Institute of Experimental Physiology and Medicine, University of Melbourne, Parkville, Vic, Australia.
Brain Res. 1994 Jul 4;650(1):40-8. doi: 10.1016/0006-8993(94)90204-6.
The distribution of angiotensin II binding sites has been mapped at segmental levels throughout the spinal cord of the sheep using in vitro autoradiographic methods. Binding of 125I-[Sar1.Ile8] Ang II is most prominent in the lateral horns of the thoracolumbar and sacral regions containing the sympathetic and parasympathetic preganglionic neurons respectively. Binding is also present in the dorsal horns of the grey matter, in the central canal region, dorsal root ganglia and associated with non-neuronal elements such as the ependyma surrounding the central canal, and blood vessels. Displacement with receptor antagonists specific for AT1 and AT2 subtypes, indicates that angiotensin II receptors in the spinal cord are of the AT1 type. These data help to interpret the physiological actions of angiotensin II in the spinal cord, particularly with respect to its autonomic components.
利用体外放射自显影方法，已在绵羊脊髓的节段水平绘制了血管紧张素II结合位点的分布图。125I-[Sar1.Ile8]血管紧张素II的结合在胸腰段和骶段的外侧角最为显著，分别包含交感和副交感神经节前神经元。结合也存在于灰质的背角、中央管区域、背根神经节，并与非神经元成分如中央管周围的室管膜和血管相关。用AT1和AT2亚型特异性受体拮抗剂进行置换表明，脊髓中的血管紧张素II受体属于AT1型。这些数据有助于解释血管紧张素II在脊髓中的生理作用，特别是其自主神经成分方面的作用。
