Bettendorff L, Hennuy B, De Clerck A, Wins P
Laboratory of Neurochemistry, University of Liège, Belgium.
Brain Res. 1994 Jul 25;652(1):157-60. doi: 10.1016/0006-8993(94)90331-x.
Incubation of rat brain homogenates with thiamine or thiamine diphosphate (TDP) leads to a synthesis of thiamine triphosphate (TTP). In membrane vesicles subsequently prepared from the homogenates, increased TTP content correlates with increased 36Cl- uptake. A hyperbolic relationship was obtained with a K0.5 of 0.27 nmol TTP/mg protein. In crude mitochondrial fractions from the brains of animals previously treated with thiamine or sulbutiamine, a positive correlation between 36Cl- uptake and TTP content was found. These results, together with other results previously obtained with the patch-clamp technique, suggest that TTP is an activator of chloride channels having a large unit conductance.
用硫胺素或硫胺二磷酸（TDP）孵育大鼠脑匀浆会导致三磷酸硫胺素（TTP）的合成。在随后从匀浆中制备的膜囊泡中，TTP含量的增加与³⁶Cl⁻摄取的增加相关。得到了一种双曲线关系，K0.5为0.27 nmol TTP/毫克蛋白质。在先前用硫胺素或舒必利处理过的动物的脑粗线粒体部分中，发现³⁶Cl⁻摄取与TTP含量之间存在正相关。这些结果，连同先前用膜片钳技术获得的其他结果，表明TTP是具有大单位电导的氯离子通道的激活剂。
