Sakata T, Kurokawa M, Oohara A, Yoshimatsu H
Department of Internal Medicine I, School of Medicine, Oita Medical University, Japan.
Brain Res Bull. 1994;35(2):135-9. doi: 10.1016/0361-9230(94)90093-0.
Histaminergic activation in the rat hypothalamus was investigated under a deficit in energy supply. Fasting of rats for 24 h increased hypothalamic histamine (HA) content. Intraperitoneal (IP) injection of insulin (2 U/kg) increased pargyline-induced accumulation of tele-methylhistamine (t-MH) leaving steady-state HA and t-MH levels unaffected, which implies enhancement of HA turnover rate. The insulin infusion induced hypoglycemia both in rats with and without pargyline pretreatment. Infusion of 2-deoxy-D-glucose (2-DG) into the third cerebroventricle also produced an increase in pargyline-induced accumulation of t-MH and no change in steady-state HA and t-MH levels. The 2-DG infusion induced hyperglycemia. Hypothalamic glycogen content decreased after 24 h starvation, but this decrease was prevented by depletion of HA by alpha-fluoromethylhistidine. Absolute glycogen contents in the cortex were lower than those in the hypothalamus, and were not affected by fasting or depletion of HA. The results indicate that activation of hypothalamic HA in response to glucoprivation may modulate homeostatic control of energy supply in the brain.
在能量供应不足的情况下，对大鼠下丘脑的组胺能激活进行了研究。大鼠禁食24小时会增加下丘脑组胺（HA）含量。腹腔注射胰岛素（2 U/kg）会增加帕吉林诱导的3-甲基组胺（t-MH）积累，而稳态HA和t-MH水平不受影响，这意味着HA周转率提高。胰岛素输注在有或没有帕吉林预处理的大鼠中均会诱导低血糖。向第三脑室注入2-脱氧-D-葡萄糖（2-DG）也会使帕吉林诱导的t-MH积累增加，而稳态HA和t-MH水平没有变化。2-DG注入会诱导高血糖。饥饿24小时后下丘脑糖原含量降低，但这种降低可通过α-氟甲基组胺消耗HA来预防。皮质中的绝对糖原含量低于下丘脑，且不受禁食或HA消耗的影响。结果表明，响应糖缺乏而下丘脑HA的激活可能会调节大脑中能量供应的稳态控制。
