Oohara A, Yoshimatsu H, Kurokawa M, Oishi R, Saeki K, Sakata T
Department of Internal Medicine I, Faculty of Medicine, Kyushu University, Fukuoka, Japan.
J Neurochem. 1994 Aug;63(2):677-82. doi: 10.1046/j.1471-4159.1994.63020677.x.
Histamine (HA) turnover in the rat hypothalamus following insufficient energy supply due to glucoprivation was examined after administration of insulin or 2-deoxy-D-glucose (2-DG). HA turnover was assessed by accumulation of tele-methylhistamine (t-MH), a major metabolite of brain HA, following administration of pargyline. Intraperitoneal injection of 1, 2, and 4 U/kg of insulin, which had no influence on steady-state levels of HA and t-MH, increased pargyline-induced accumulation of t-MH. Accumulation of t-MH due to pargyline was inversely related to the concomitant plasma glucose concentration after different doses of insulin. The level of t-MH accumulated by pargyline did not change compared with that of controls, when a euglycemic condition was maintained or insulin at a dose of 6 mU per rat was infused into the third cerebroventricle. Intracerebroventricular infusion of 24 mumol per rat of 2-DG, which had no influence on steady-state levels of HA and t-MH, increased the level of t-MH enhanced by pargyline. The results indicate that an increase in hypothalamic HA turnover in response to glucoprivation may be involved in homeostatic regulation of energy metabolism in the brain.
在给予胰岛素或2-脱氧-D-葡萄糖（2-DG）后，研究了因糖剥夺导致能量供应不足的大鼠下丘脑组胺（HA）周转率。通过给予帕吉林后脑HA的主要代谢产物3-甲基组胺（t-MH）的积累来评估HA周转率。腹腔注射1、2和4 U/kg的胰岛素，这对HA和t-MH的稳态水平没有影响，但增加了帕吉林诱导的t-MH积累。不同剂量胰岛素后，帕吉林引起的t-MH积累与同时的血浆葡萄糖浓度呈负相关。当维持血糖正常状态或将每只大鼠6 mU剂量的胰岛素注入第三脑室时，帕吉林积累的t-MH水平与对照组相比没有变化。向脑室内注入每只大鼠24 μmol的2-DG，这对HA和t-MH的稳态水平没有影响，但增加了帕吉林增强的t-MH水平。结果表明，对糖剥夺的反应中下丘脑HA周转率的增加可能参与了大脑能量代谢的稳态调节
