Kowalczyk D, Skorupski W, Drews M, Nowak J
Institute of Human Genetics, Polish Academy of Sciences, Poznań.
Cancer Immunol Immunother. 1994 Oct;39(4):275-8. doi: 10.1007/BF01525992.
Tumour-infiltrating lymphocytes (TIL) and peripheral blood lymphocytes (PBL) from four patients with renal-cell carcinoma (three paired with blood), two colon carcinomas (both paired with blood) and two melanomas (blood was not available) were analysed for the T cell receptor (TCR) delta gene repertoire. Polymerase chain reaction analysis, employing a panel of specific primers for TCR delta gene segments, showed different gene rearrangement patterns in TIL and PBL in all patients. Simultaneous analysis of TIL and PBL revealed the presence of lymphoid cells in the tumour tissue that were not present in the periphery. These results demonstrate that, although tumour-infiltrating lymphocytes contain gamma/delta T cells within the range observed in peripheral blood, these cells differ from those in peripheral blood in their gene repertoire and this may account for selective accumulation or/and in situ amplification of gamma/delta lymphocytes at the tumour site, indicating a unique type of host reaction against tumour.
对4例肾细胞癌患者（3例有配对血液样本）、2例结肠癌患者（均有配对血液样本）和2例黑色素瘤患者（无血液样本）的肿瘤浸润淋巴细胞（TIL）和外周血淋巴细胞（PBL）进行了T细胞受体（TCR）δ基因库分析。采用一组针对TCR δ基因片段的特异性引物进行聚合酶链反应分析，结果显示所有患者的TIL和PBL中存在不同的基因重排模式。对TIL和PBL的同步分析揭示，肿瘤组织中存在外周血中不存在的淋巴细胞。这些结果表明，尽管肿瘤浸润淋巴细胞中γ/δ T细胞的含量在在外周血中观察到的范围内，但这些细胞的基因库与外周血中的不同，这可能解释了γ/δ淋巴细胞在肿瘤部位的选择性积聚或/和原位扩增，表明宿主对肿瘤存在一种独特的反应类型。
