Yamasaki K, Hashimoto A, Kokusenya Y, Miyamoto T, Sato T
Analytical Chemistry Research Laboratory, Tanabe Seiyaku Co., Ltd., Osaka, Japan.
Chem Pharm Bull (Tokyo). 1994 Aug;42(8):1663-5. doi: 10.1248/cpb.42.1663.
The antioxidative effects of methanol extracts of crude drugs were estimated by an electrochemical method because there are many electrochemically-active substances in natural antioxidants. Twelve kinds of crude drugs, which had been reported to exhibit strong activity in an antioxidative test based on the air oxidation of linoleic acid, were studied. The oxidative capacity calculated from voltammograms of their methanol extracts were compared and examined together with data on their radical scavenging effects. The results showed that the electrochemical behavior in most cases correlated with the radical scavenging effect. Crude drugs which had clear oxidative peaks below +1.2 V and a large oxidative capacity were suggested to have strong radical scavenging effects. It was clear that substances oxidized at lower potentials had stronger radical scavenging effects than those oxidized at higher potentials. Therefore, this electrochemical method can be considered as a rapid and simple method for estimating the antioxidative effects as a radical scavenger.
由于天然抗氧化剂中存在许多具有电化学活性的物质，因此采用电化学方法评估了生药甲醇提取物的抗氧化作用。研究了12种生药，这些生药在基于亚油酸空气氧化的抗氧化试验中表现出较强的活性。比较并检查了从其甲醇提取物的伏安图计算出的氧化能力以及它们的自由基清除效果数据。结果表明，在大多数情况下，电化学行为与自由基清除效果相关。在+1.2 V以下具有清晰氧化峰且氧化能力较大的生药被认为具有较强的自由基清除作用。很明显，在较低电位下被氧化的物质比在较高电位下被氧化的物质具有更强的自由基清除作用。因此，这种电化学方法可被视为一种快速、简单的评估作为自由基清除剂的抗氧化作用的方法。
