Chatatikun Moragot, Chiabchalard Anchalee
Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, Thailand.
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
BMC Complement Altern Med. 2017 Nov 9;17(1):487. doi: 10.1186/s12906-017-1994-7.
Ultraviolet radiation from sunlight induces overproduction of reactive oxygen species (ROS) resulting in skin photoaging and hyperpigmentation disorders. Novel whitening and anti-wrinkle compounds from natural products have recently become of increasing interest. The purpose of this study was to find products that reduce ROS in 14 Thai plant extracts.
To determine total phenolic and flavonoid content, antioxidant activity, anti-tyrosinase activity and anti-collagenase activity, we compared extracts of 14 Thai plants prepared using different solvents (petroleum ether, dichloromethane and ethanol). Antioxidant activities were determined by DPPH and ABTS assays.
Total phenolic content of the 14 Thai plants extracts was found at the highest levels in ethanol followed by dichloromethane and petroleum ether extracts, respectively, while flavonoid content was normally found in the dichloromethane fraction. Scavenging activity ranged from 7 to 99% scavenging as assessed by DPPH and ABTS assays. The ethanol leaf extract of Ardisia elliptica Thunb. had the highest phenolic content, antioxidant activity and collagenase inhibition, while Cassia alata (L.) Roxb. extract had the richest flavonoid content. Interestingly, three plants extracts, which were the ethanolic fractions of Annona squamosa L., Ardisia elliptica Thunb. and Senna alata (L.) Roxb., had high antioxidant content and activity, and significantly inhibited both tyrosinase and collagenase.
Our finding show that the ethanol fractions of Annona squamosa L., Ardisia elliptica Thunb. and Senna alata (L.) Roxb. show promise as potential ingredients for cosmetic products such as anti-wrinkle agents and skin whitening products.
阳光中的紫外线会诱导活性氧（ROS）过度产生，从而导致皮肤光老化和色素沉着紊乱。近年来，来自天然产物的新型美白和抗皱化合物越来越受到关注。本研究的目的是在14种泰国植物提取物中寻找能降低ROS的产品。
为了测定总酚和黄酮含量、抗氧化活性、抗酪氨酸酶活性和抗胶原酶活性，我们比较了使用不同溶剂（石油醚、二氯甲烷和乙醇）制备的14种泰国植物的提取物。通过DPPH和ABTS法测定抗氧化活性。
14种泰国植物提取物的总酚含量在乙醇提取物中最高，其次是二氯甲烷提取物和石油醚提取物，而黄酮含量通常在二氯甲烷馏分中。通过DPPH和ABTS法评估，清除活性范围为7%至99%。椭圆叶紫金牛的乙醇叶提取物酚含量、抗氧化活性和胶原酶抑制作用最高，而翅荚决明提取物的黄酮含量最丰富。有趣的是，三种植物提取物，即番荔枝、椭圆叶紫金牛和番泻叶的乙醇馏分，具有高抗氧化剂含量和活性，并能显著抑制酪氨酸酶和胶原酶。
我们的研究结果表明，番荔枝、椭圆叶紫金牛和番泻叶的乙醇馏分有望作为抗皱剂和皮肤美白产品等化妆品的潜在成分。
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