Suthisamphat Naranpraphai, Dechayont Bhanuz, Phuaklee Pathompong, Prajuabjinda Onmanee, Vilaichone Ratha-Korn, Itharat Arunporn, Mokmued Khwanchanok, Prommee Nuntika
Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
Gastroenterology Unit, Department of Medicine, Thammasat University, Pathum Thani 12120, Thailand.
Evid Based Complement Alternat Med. 2020 Mar 29;2020:7576818. doi: 10.1155/2020/7576818. eCollection 2020.
The aril (mace) of , known as Dok-Chan, is a spice that has long been used for treating stomach discomfort, peptic ulcer, and nausea. It is an ingredient in many remedies in Thai traditional medicine, e.g., Ya-Hom-Thep-Bha-Jit, Ya-Hom-Nao-Wa-Kot, and Ya-That-Bun-Job, which are used to treat dyspepsia and other gastrointestinal tract symptoms. The aqueous and ethanolic extracts of mace were used for all tests. Anti activities were determined by the disc diffusion method and agar dilution. Anti-inflammatory activity was determined by the LPS-induced nitric oxide (NO) inhibition in a RAW264.7 cell line, and cytotoxicity was determined against gastric cancer cell lines (Kato III) using the sulphorhodamine B (SRB) assay. The DPPH radical scavenging and ABTS radical cation decolorization assays were used to determine the antioxidant activities. The result found that the ethanolic extract of mace exhibited antimicrobial activity against ATCC 43504 and six clinical strains with MIC values of 125-250 g/ml. The aqueous extract MICs against ATCC reference strain and six clinical strains were 500 g/ml compared with 0.5 g/ml for the positive control, clarithromycin. The inhibitory effect of LPS-induced NO release and cytotoxic activity of the ethanolic extract had IC values of 82.19 g/ml and 26.06 g/ml, respectively, and the EC values for the DPPH and ABTS antioxidant assays were 13.41 g/ml and 12.44 g/ml, respectively. The mace extract also had anticancer properties. In conclusion, the ethanolic mace extract had anti-, anti-inflammatory, antioxidant, and anticancer activities. These data support further preclinical and clinical investigation to see if the mace extract could have a role in treating patients with dyspepsia, peptic ulcers, and possibly gastric cancer.
肉豆蔻的假种皮（肉豆蔻衣），被称为“Dok-Chan”，是一种长期用于治疗胃部不适、消化性溃疡和恶心的香料。它是泰国传统医学中许多药物的成分，例如用于治疗消化不良和其他胃肠道症状的Ya-Hom-Thep-Bha-Jit、Ya-Hom-Nao-Wa-Kot和Ya-That-Bun-Job。肉豆蔻衣的水提取物和乙醇提取物用于所有测试。抗菌活性通过纸片扩散法和琼脂稀释法测定。抗炎活性通过RAW264.7细胞系中脂多糖诱导的一氧化氮（NO）抑制来测定，细胞毒性通过使用磺酰罗丹明B（SRB）测定法针对胃癌细胞系（Kato III）进行测定。DPPH自由基清除和ABTS自由基阳离子脱色测定法用于测定抗氧化活性。结果发现，肉豆蔻衣的乙醇提取物对ATCC 43504和六种临床菌株具有抗菌活性，MIC值为125-250μg/ml。水提取物对ATCC参考菌株和六种临床菌株的MIC值为500μg/ml，而阳性对照克拉霉素的MIC值为0.5μg/ml。乙醇提取物对脂多糖诱导的NO释放的抑制作用和细胞毒性活性的IC值分别为82.19μg/ml和26.06μg/ml，DPPH和ABTS抗氧化测定的EC值分别为13.41μg/ml和12.44μg/ml。肉豆蔻衣提取物还具有抗癌特性。总之，乙醇肉豆蔻衣提取物具有抗菌、抗炎、抗氧化和抗癌活性。这些数据支持进一步的临床前和临床研究，以确定肉豆蔻衣提取物是否可用于治疗消化不良、消化性溃疡以及可能的胃癌患者。
