Dai W, Liu P, Han Y
Institute of Basic Medical Sciences, CAMS and PUMC, Beijing.
Zhongguo Yi Xue Ke Xue Yuan Xue Bao. 1994 Feb;16(1):20-3.
Rat epididymal sperms were collected after 7 weeks of treatment with Tripterygium wilfordii monomers T4,T7,T15 and triptolide. Total nuclear basic protein (TNBP) were extracted from sperm nuclei isolated by sonication. The relative proportions of histones and protamine were determined by scanning microdensitometry following electrophoresis of TNBP in polyacrylamide gels. It was found that the content of TNBP was reduced while the total histone/protamine ratios were increased following treatment, indicating a marked decrease of protamine levels as compared with the control group. These results suggest that the interruption of nuclear protein transition of spermatids induced by T4,T7,T15 and triptolide might lead to infertility.
用雷公藤单体T4、T7、T15和雷公藤内酯醇处理7周后，收集大鼠附睾精子。通过超声处理分离精子细胞核，从中提取总核碱性蛋白（TNBP）。在聚丙烯酰胺凝胶中对TNBP进行电泳后，通过扫描显微密度测定法确定组蛋白和鱼精蛋白的相对比例。结果发现，处理后TNBP含量降低，而总组蛋白/鱼精蛋白比例增加，表明与对照组相比，鱼精蛋白水平显著降低。这些结果表明，T4、T7、T15和雷公藤内酯醇诱导的精子细胞核蛋白转变中断可能导致不育。
