Zhan B, Zhang L, Wang J, Feng X
Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(2):111-4.
A pair of primers were designed and synthesized based on the DNA fragment coded erythrocyte-binding antigen (EBA-175) of Plasmodium falciparum (P.f.) and polymerase chain reaction (PCR) was performed to detect P.f. cultured in vitro. The amplified products were analysed by agarose gel electrophoresis and a specific 492 bp DNA band was showed in P.f. infected blood sample, but no band showed in P. vivax, P. cynomolgi, P. yoelii and P. berghei infected blood samples, nor in normal one. The parasite densities detected by this method could be as minimal as 10 parasites/20 microliters blood. The PCR technique showed high sensitivity and specificity.
根据恶性疟原虫编码红细胞结合抗原（EBA - 175）的DNA片段设计并合成了一对引物，采用聚合酶链反应（PCR）检测体外培养的恶性疟原虫。扩增产物经琼脂糖凝胶电泳分析，结果显示恶性疟原虫感染的血液样本中有一条492 bp的特异性DNA条带，而间日疟原虫、食蟹猴疟原虫、约氏疟原虫和伯氏疟原虫感染的血液样本以及正常血液样本中均未出现条带。该方法检测到的疟原虫密度可低至每20微升血液中有10个疟原虫。PCR技术显示出高灵敏度和特异性。
