[通过聚合酶链反应诊断恶性疟原虫疟疾]

[Diagnosis of falciparum malaria by polymerase chain reaction].

作者信息

Zhan B, Zhang L X, Wang J J, Feng X P

机构信息

Institute of Parasitic Diseases, Chinese Academy of Preventive Medicine, Shanghai.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(1):37-9.

PMID:8044903

Abstract

Polymerase Chain Reaction (PCR) method using a pair of Plasmodium falciparum-specific oligonucleotides as primers was used to detect malaria patient blood samples collected from Yunnan endemic malarious area. The results showed that a DNA fragment of 492 bp was amplified in all 53 microscopically diagnosed P. falciparum samples, but negative in 8 P. vivax samples and 12 normal blood samples. This method seems to have high sensitivity and high specificity.

摘要

采用一对恶性疟原虫特异性寡核苷酸作为引物的聚合酶链反应（PCR）方法，对从云南疟疾流行区采集的疟疾患者血样进行检测。结果显示，在所有53份经显微镜诊断为恶性疟原虫的样本中均扩增出一条492 bp的DNA片段，但在8份间日疟原虫样本和12份正常血样中呈阴性。该方法似乎具有高灵敏度和高特异性。