[应用固相聚合酶链反应检测恶性疟原虫特异性DNA片段]
[Detection of specific DNA fragment of Plasmodium falciparum by solid phase PCR].
作者信息
Yu Y, He J, Cai L, Ye F, Xin H
机构信息
Department of Clinical Laboratory, Changhai Hospital, Second Military Medical University.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(2):122-4.
Solid phase PCR for detecting Plasmodium falciparum was established and used to detect cultured FCCl/HN isolate and malaria patients in Yunnan Province. The results revealed that the sensitivity of the method for detecting FCCl/HN isolate DNA was as minimal as 0.2 pg, or about 10 parasites. The specificity of the method was confirmed by discriminating malaria patients infected with different species of Plasmodium. The results suggest that solid phase PCR is a specific and sensitive method for early detection of P. falciparum.
建立了用于检测恶性疟原虫的固相PCR方法，并用于检测培养的FCC1/HN分离株和云南省的疟疾患者。结果显示，该方法检测FCC1/HN分离株DNA的灵敏度低至0.2 pg，约为10个疟原虫。通过区分感染不同疟原虫种类的疟疾患者，证实了该方法的特异性。结果表明，固相PCR是早期检测恶性疟原虫的一种特异且灵敏的方法。
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