Beheshti I, Wessels L M, Eckfeldt J H
Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis 55455-0392.
Clin Chem. 1994 Nov;40(11 Pt 1):2088-92.
To investigate EDTA-plasma/serum (P/S) differences, we collected paired samples from 25 volunteers and measured total cholesterol (TC), triglyceride (TG) and high-density-lipoprotein cholesterol (HDLC), using the Cobas FARA, Ektachem 700, DuPont Dimension, and Baxter Paramax Analyzers. The mean (SD) P/S ratios for TC, HDLC, and TG concentrations were, respectively: 0.980 (0.0171), 1.063 (0.0704), and 0.961 (0.363) for Paramax; 0.976 (0.0189), 1.034 (0.1091), and 0.950 (0.557) for Dimension; 1.003 (0.0221), 1.059 (0.0304), and 0.988 (0.0179) for Ektachem; and 0.993 (0.0162), 1.063 (0.0830), and 1.013 (0.0410) for Cobas. We conclude that P/S ratios vary by analytical methods, and that HDLC ratios tend to be larger in magnitude and in the opposite direction from TC and TG. Both effects lead to significant biases in computed disease risk.
为研究乙二胺四乙酸（EDTA）血浆/血清（P/S）差异，我们从25名志愿者处采集了配对样本，并使用罗氏Cobas FARA、拜耳Ektachem 700、杜邦Dimension和百特Baxter Paramax分析仪测量了总胆固醇（TC）、甘油三酯（TG）和高密度脂蛋白胆固醇（HDLC）。Paramax分析仪测得的TC、HDLC和TG浓度的平均（标准差）P/S比值分别为：0.980（0.0171）、1.063（0.0704）和0.961（0.363）；Dimension分析仪测得的分别为：0.976（0.0189）、1.034（0.1091）和0.950（0.557）；Ektachem分析仪测得的分别为：1.003（0.0221）、1.059（0.0304）和0.988（0.0179）；Cobas分析仪测得的分别为：0.993（0.0162）、1.063（0.0830）和1.013（0.0410）。我们得出结论，P/S比值因分析方法而异，且HDLC比值在幅度上往往更大，且与TC和TG的方向相反。这两种效应都会导致计算出的疾病风险出现显著偏差。
