Cash E, Rott O
INSERM U283, Cochin Hospital, Paris, France.
Clin Exp Immunol. 1994 Nov;98(2):313-8. doi: 10.1111/j.1365-2249.1994.tb06143.x.
The potential of central nervous system (CNS)-derived cells for initiating T cell responses is not known. Using the capacity of unprimed T cells to respond to allogeneic determinants on antigen-presenting cells (APC), we assessed the ability of microglial cells to act as stimulators of primary T cell responses in vitro. For this purpose, microglial cells were activated with lipopolysaccharide (LPS), interferon-gamma (IFN-gamma), or by phagocytosis of progenitor oligodendrocytes and subsequently tested for their ability to induce a proliferative response of naive, resting T cells. Activated microglial cells induced a significant proliferation of virgin, alloreactive CD4+ and CD8+ T lymphocytes, with a more substantial response of highly purified CD4+ than of CD8-expressing T cells. Phagocytosis activation was the most efficient stimulus to induce this APC competence on microglial cells. By contrast, IFN-gamma-pretreated, MHC-expressing astrocytes were unable to induce similar responses of alloreactive CD4+ or CD8+ T cells under the same experimental conditions. Collectively, our data suggest the role of activated microglia as the fully immunocompetent accessory cell population of the CNS.
中枢神经系统（CNS）来源的细胞引发T细胞反应的潜力尚不清楚。利用未致敏T细胞对抗抗原呈递细胞（APC）上同种异体决定簇作出反应的能力，我们评估了小胶质细胞在体外作为原发性T细胞反应刺激物的能力。为此，用脂多糖（LPS）、干扰素-γ（IFN-γ）或通过吞噬祖少突胶质细胞激活小胶质细胞，随后测试它们诱导未活化、静止T细胞增殖反应的能力。活化的小胶质细胞诱导了未致敏、同种异体反应性CD4+和CD8+ T淋巴细胞的显著增殖，高度纯化的CD4+ T细胞的反应比表达CD8的T细胞更强烈。吞噬激活是诱导小胶质细胞这种APC能力的最有效刺激。相比之下，在相同实验条件下，经IFN-γ预处理、表达MHC的星形胶质细胞无法诱导同种异体反应性CD4+或CD8+ T细胞产生类似反应。总体而言，我们的数据表明活化的小胶质细胞作为CNS具有完全免疫活性的辅助细胞群体的作用。
