Walker W S, Gatewood J, Olivas E, Askew D, Havenith C E
Department of Immunology, St. Jude Children's Research Hospital, Memphis, TN 3810, USA.
J Neuroimmunol. 1995 Dec 31;63(2):163-74. doi: 10.1016/0165-5728(95)00146-8.
We developed a panel of non-virus transformed cell lines derived from individual microglial precursors residing in the brains of normal mice. These colony stimulating factor-1-dependent cell lines are B7-1+ (CD80), Mac-1+, Mac-2+, Mac-3+, CD45+, MHC class I+, colony stimulating factor-1 receptor+, and they ingest antibody-coated particles. However, the cell lines differ in their expression of B7-2 (CD86), F4/80, Ly-6C and MHC class II molecules. They also differ in their ability to constitutively process and present antigens to naive CD4+ and CD8+ T cells, memory CD4+ and CD8+, and in the manner by which interferon gamma modulates their antigen-presenting activities. These cell lines should be valuable as models for studies on the immunobiology of the microglia.
我们从正常小鼠大脑中分离出单个小胶质前体细胞，建立了一组非病毒转化的细胞系。这些依赖集落刺激因子-1的细胞系表达B7-1+(CD80)、Mac-1+、Mac-2+、Mac-3+、CD45+、MHC I类分子、集落刺激因子-1受体，并且能够摄取抗体包被的颗粒。然而，这些细胞系在B7-2(CD86)、F4/80、Ly-6C和MHC II类分子的表达上存在差异。它们在组成性加工和呈递抗原给初始CD4+和CD8+T细胞、记忆性CD4+和CD8+T细胞的能力上也有所不同，并且在γ干扰素调节其抗原呈递活性的方式上也存在差异。这些细胞系作为小胶质细胞免疫生物学研究的模型应该具有重要价值。
