Letson A K, Dahners L E
University of North Carolina at Chapel Hill.
Clin Orthop Relat Res. 1994 Nov(308):207-12.
Platelet-derived growth factor, basic fibroblast growth factor, and insulin-like growth factor Type 1 have demonstrated chemotactic and mitogenic properties in vitro that could benefit healing ligaments. These growth factors also have demonstrated in vivo effects when used in skin wound-healing models. In a previous study, bFGF and IGF-1, used alone, failed to improve healing. The authors wished to test the hypothesis that platelet-derived growth factor alone or combinations of platelet-derived growth factor plus insulin-like growth factor Type 1, platelet-derived growth factor plus basic fibroblast growth factor, or basic fibroblast growth factor plus insulin-like growth factor Type 1 would increase the healing efficiency of ligaments because of their effects on different parts of the cell replication cycle and their abilities to induce chemotaxis. Ligaments receiving platelet-derived growth factor alone were 73% +/- 55% stronger than their contralateral internal controls (p < 0.0025). Stiffness increased 94% +/- 63% over controls (p < 0.0025), and breaking energy was 101% +/- 104% > controls (p < 0.1). Ligaments treated with a combination of platelet-derived growth factor plus insulin-like growth factor Type 1 and platelet-derived growth factor plus basic fibroblast growth factor also had increases in rupture force, stiffness, and breaking energy over internal controls. Ligaments treated with a combination of basic fibroblast growth factor plus insulin-like growth factor Type 1 also appeared to have some improved healing; the results, however, did not prove to be statistically significant. Synergistic effects of growth factor combinations were not evident.
血小板衍生生长因子、碱性成纤维细胞生长因子和胰岛素样生长因子1在体外已显示出趋化和促有丝分裂特性，可能有益于韧带愈合。这些生长因子在皮肤伤口愈合模型中使用时也已显示出体内效应。在先前的一项研究中，单独使用碱性成纤维细胞生长因子和胰岛素样生长因子1未能改善愈合情况。作者希望检验以下假设：单独使用血小板衍生生长因子或血小板衍生生长因子与胰岛素样生长因子1的组合、血小板衍生生长因子与碱性成纤维细胞生长因子的组合，或碱性成纤维细胞生长因子与胰岛素样生长因子1的组合，由于它们对细胞复制周期不同部分的影响以及诱导趋化的能力，会提高韧带的愈合效率。单独接受血小板衍生生长因子治疗的韧带比其对侧的内部对照强73%±55%（p<0.0025）。刚度比对照增加了94%±63%（p<0.0025），断裂能量比对照高101%±104%（p<0.1）。用血小板衍生生长因子与胰岛素样生长因子1的组合以及血小板衍生生长因子与碱性成纤维细胞生长因子的组合治疗的韧带，其破裂力、刚度和断裂能量也比内部对照有所增加。用碱性成纤维细胞生长因子与胰岛素样生长因子1的组合治疗的韧带似乎也有一些愈合改善；然而，结果并未证明具有统计学意义。生长因子组合的协同效应不明显。
