Yoshikawa H, Shimizu K, Nakase T, Takaoka K
Department of Orthopaedic Surgery, Osaka University Medical School, Suita, Japan.
Clin Orthop Relat Res. 1994 Nov(308):213-9.
Periosteal sunburst spiculation is a peculiar radiographic feature of osteosarcoma, and it represents a reactive ossification resulting from the action of normal osteoblasts rather than tumor cells. Because bone morphogenetic protein is known to be a potent inducer of ectopic bone formation, the authors hypothesized that bone morphogenetic protein may be involved in the pathogenesis of such reactive bone formation in osteosarcoma. Chinese hamster ovary cells transfected with the bone morphogenetic protein-4 gene were injected into the femurs of athymic nude mice to form experimental bone tumors producing bone morphogenetic protein. Two weeks after intramedullary injection, new bone formation was observed radiographically and histologically within the extraosseous portions of the tumors. This showed a close resemblance to sunburst spiculation in human osteosarcomas. In contrast, the control nontransformed Chinese hamster ovary tumors showed no extraosseous bone formation. Because the induced bone was composed of multiple parallel spicules similar to those found in human bone morphogenetic protein-producing osteosarcomas, these findings suggest that periosteal sunburst spiculation may be the result of bone morphogenetic protein production by osteosarcoma cells.
骨膜日光放射状骨针是骨肉瘤一种独特的影像学特征，它代表由正常成骨细胞而非肿瘤细胞作用导致的反应性骨化。由于已知骨形态发生蛋白是异位骨形成的强效诱导剂，作者推测骨形态发生蛋白可能参与骨肉瘤中这种反应性骨形成的发病机制。将转染了骨形态发生蛋白-4基因的中国仓鼠卵巢细胞注射到无胸腺裸鼠的股骨中，以形成产生骨形态发生蛋白的实验性骨肿瘤。髓内注射两周后，在肿瘤的骨外部分在影像学和组织学上观察到新骨形成。这与人类骨肉瘤中的日光放射状骨针非常相似。相比之下，对照未转化的中国仓鼠卵巢肿瘤未显示骨外骨形成。由于诱导形成的骨由多个平行骨针组成，类似于在产生骨形态发生蛋白的人类骨肉瘤中发现的骨针，这些发现表明骨膜日光放射状骨针可能是骨肉瘤细胞产生骨形态发生蛋白的结果。
