Yoshikawa H, Takaoka K, Hamada H, Ono K
Cancer. 1985 Oct 1;56(7):1682-7. doi: 10.1002/1097-0142(19851001)56:7<1682::aid-cncr2820560735>3.0.co;2-8.
Bone morphogenetic activity of osteosarcomas from 20 patients was assayed. The activity was demonstrated as ectopic bone formation on implantation of a lyophilized fraction of the tumor into athymic nude mice in 8 of 20 cases. Osteosarcomas producing bone morphogenetic protein (BMP) differed in clinical features from those not producing BMP. They were characterized radiologically by perpendicular spicules, histologically by osteoblastic type cells, and clinically by an increased serum alkaline phosphatase level, relative resistance to preoperative chemotherapy with Adriamycin (doxorubicin) plus high-dose methotrexate, and a tendency to metastasize to other bones and the lungs.
对20例骨肉瘤患者的骨形态发生活性进行了测定。在20例病例中的8例中，将肿瘤的冻干部分植入无胸腺裸鼠后，该活性表现为异位骨形成。产生骨形态发生蛋白（BMP）的骨肉瘤在临床特征上与不产生BMP的骨肉瘤不同。它们在放射学上的特征是垂直的骨针，组织学上的特征是成骨细胞类型的细胞，临床上的特征是血清碱性磷酸酶水平升高、对阿霉素（多柔比星）加高剂量甲氨蝶呤的术前化疗相对耐药，以及有转移至其他骨骼和肺部的倾向。
