Zwijsen A, Blockx H, Van Arnhem W, Willems J, Fransen L, Devos K, Raymackers J, Van de Voorde A, Slegers H
Department of Biochemistry, University of Antwerp, Belgium.
Eur J Biochem. 1994 Nov 1;225(3):937-46. doi: 10.1111/j.1432-1033.1994.0937b.x.
A protein was isolated from rat C6 glioma-conditioned medium and was biochemically characterized. The heparin-binding protein has a native molecular mass of 55-75,000 Da, a molecular mass of 40-48,000 Da under denaturing conditions, and a pI of 5.0-6.0. Based on the determined partial amino acid sequences, the full lenght cDNA encoding the rat and human proteins were cloned. The cDNA sequences identified the isolated rat and human protein as the homologue of a recently reported mouse osteoblast-transforming-growth-factor-beta 1-inducible protein, encoded by the TSC-36 gene [Shibanuma, M., Mashimo, J., Mita, A., Kuroki, T. & Nose, K. (1993) Eur. J. Biochem. 217, 13-19]. Analysis of the human, rat and mouse amino acid sequences indicates that these proteins are highly conserved (> 92% sequence identity). Sequence similarities with follistatin and the follistatin-like domain of agrin are revealed. The relationship with follistatin and agrin points to possible common functions for the cloned follistatin-related proteins (FRP). The protein has no effect on the inhibitory action of transforming growth factor-beta 1, on CCl-64 cell growth.
从大鼠C6胶质瘤条件培养基中分离出一种蛋白质，并对其进行了生化特性分析。该肝素结合蛋白的天然分子量为55 - 75,000 Da，变性条件下分子量为40 - 48,000 Da，pI为5.0 - 6.0。根据所确定的部分氨基酸序列，克隆了编码大鼠和人类蛋白质的全长cDNA。cDNA序列确定分离出的大鼠和人类蛋白质是最近报道的由TSC - 36基因编码的小鼠成骨细胞转化生长因子-β1诱导蛋白的同源物[柴沼，M.，益茂，J.，三田，A.，黑木，T. & 野泽，K.（1993年）《欧洲生物化学杂志》217卷，13 - 19页]。对人类、大鼠和小鼠氨基酸序列的分析表明，这些蛋白质高度保守（序列同一性> 92%）。揭示了与卵泡抑素和聚集蛋白的卵泡抑素样结构域的序列相似性。与卵泡抑素和聚集蛋白的关系表明克隆的卵泡抑素相关蛋白（FRP）可能具有共同功能。该蛋白质对转化生长因子-β1对CCl - 64细胞生长的抑制作用没有影响。
