Fang S, Ledlow A, Murray J A, Christensen J, Conklin J L
Department of Internal Medicine, College of Medicine, University of Iowa, Iowa City.
Gastroenterology. 1994 Dec;107(6):1621-6. doi: 10.1016/0016-5085(94)90800-1.
BACKGROUND/AIMS: Vasoactive intestinal contractor (VIC), an endothelinlike peptide and a putative gastrointestinal hormone, contracts gastrointestinal smooth muscle. The aim was to study VIC in relation to esophageal function.
Intramural nerves in opossum esophageal smooth muscle strips were stimulated in the presence of various concentrations of VIC and were stained for VIC immunoreactivity.
VIC caused an atropine-resistant increase in the amplitude of nerve-induced contractions of the circular muscle. VIC alone contracted longitudinal muscle, and this effect was nearly eliminated by 1 mmol/L atropine. VIC caused an atropine-resistant increase in the resting tone of the lower esophageal sphincter muscle, but it did not affect nerve-induced relaxation of that muscle. VIC-immunoreactive nerve fibers occurred in the longitudinal muscle layer, in the muscularis mucosae, and around the ducts of esophageal glands. A few such fibers were found in the circular muscle layer. Nerve fibers and cell bodies of the myenteric plexus showed VIC immunoreactivity. In the stomach, immunoreactive nerve fibers occurred in muscularis mucosae and circular muscle but not in longitudinal muscle.
VIC is localized in neuronal elements of the opossum esophagus and excites contractions in esophageal smooth muscle.
背景/目的：血管活性肠收缩肽（VIC）是一种类内皮素肽和一种假定的胃肠激素，可使胃肠平滑肌收缩。本研究旨在探讨VIC与食管功能的关系。
在不同浓度的VIC存在下刺激负鼠食管平滑肌条中的壁内神经，并对其进行VIC免疫反应性染色。
VIC导致环行肌神经诱导收缩幅度出现阿托品抵抗性增加。单独使用VIC可使纵行肌收缩，且这种作用在1 mmol/L阿托品作用下几乎完全消除。VIC导致食管下括约肌静息张力出现阿托品抵抗性增加，但不影响该肌肉的神经诱导舒张。VIC免疫反应性神经纤维出现在纵行肌层、黏膜肌层以及食管腺导管周围。在环行肌层发现少量此类纤维。肌间神经丛的神经纤维和细胞体显示VIC免疫反应性。在胃中，免疫反应性神经纤维出现在黏膜肌层和环行肌，但不出现在纵行肌。
VIC定位于负鼠食管的神经元成分中，并可激发食管平滑肌收缩。
