Chachaty E, Saulnier P, Martin A, Mario N, Andremont A
Laboratoire d'Ecologie Microbienne, Institut Gustave-Roussy, Villejuif, France.
FEMS Microbiol Lett. 1994 Sep 15;122(1-2):61-8. doi: 10.1111/j.1574-6968.1994.tb07144.x.
Clostridium difficile is a Gram-positive sporulating anaerobic bacillus which causes pseudomembranous colitis. Nosocomial acquisition of this bacteria has proved frequent, and epidemiological markers are needed to recognize and control common-source outbreaks. We therefore compared the results of pulsed-field gel electrophoresis (PFGE) after restriction with SmaI or NruI, random-amplified polymorphic DNA (RAPD) using 3 10-mer oligonucleotides, and ribotyping to differentiate between 30 unrelated strains of C. difficile belonging to 8 serotypes. The strains were separated into 26 different types by PFGE, 25 by RAPD, but into only 18 types by ribotyping. Median percentages of similarity between strains ranged from 27 in the PFGE assay to 90 in the ribotyping assay, but there was good agreement between the 3 methods for the clustering of strains. PFGE was more time-consuming than RAPD but its patterns were easier to analyze.
艰难梭菌是一种革兰氏阳性、形成芽孢的厌氧杆菌，可引起假膜性结肠炎。已证实该细菌在医院内的感染很常见，因此需要流行病学标志物来识别和控制共同来源的暴发。为此，我们比较了用SmaI或NruI酶切后的脉冲场凝胶电泳（PFGE）、使用3种10聚体寡核苷酸的随机扩增多态性DNA（RAPD）以及核糖体分型的结果，以区分属于8种血清型的30株无关艰难梭菌菌株。PFGE将这些菌株分为26种不同类型，RAPD分为25种，而核糖体分型仅分为18种。菌株之间的相似性中位数百分比在PFGE分析中为27%，在核糖体分型分析中为90%，但这3种方法在菌株聚类方面有很好的一致性。PFGE比RAPD更耗时，但其图谱更容易分析。
