Meyer B E, Malim M H
Howard Hughes Medical Institute, University of Pennsylvania School of Medicine, Philadelphia 19104-6148.
Genes Dev. 1994 Jul 1;8(13):1538-47. doi: 10.1101/gad.8.13.1538.
The HIV-1 Rev protein is a nuclear trans-activator essential for the transport of unspliced viral transcripts to the cytoplasm. In this paper we demonstrate that Rev, rather than being confined to the nucleus, is constantly shuttling between the nucleus and the cytoplasm. We also show that inactivation of Rev's leucine-rich activation domain generates mutant proteins that not only fail to induce the nuclear export of viral transcripts but are also unable to enter the cytoplasm. On the basis of this correlation, we propose that Rev activates viral mRNA transport by directly binding to these RNAs and translocating, with them, to the cytoplasm. In addition, these results also identify, for the first time, a peptide sequence that is important for nuclear export.
HIV-1 Rev蛋白是一种核反式激活因子，对于未剪接的病毒转录本转运至细胞质至关重要。在本文中，我们证明Rev并非局限于细胞核内，而是在细胞核与细胞质之间不断穿梭。我们还表明，Rev富含亮氨酸的激活结构域失活会产生突变蛋白，这些蛋白不仅无法诱导病毒转录本的核输出，而且也无法进入细胞质。基于这种相关性，我们提出Rev通过直接结合这些RNA并与它们一起转运至细胞质来激活病毒mRNA的转运。此外，这些结果还首次鉴定出了一个对核输出很重要的肽序列。
