2-二苯基甲基硅烷基乙基（DPSE）：一种用于寡脱氧核糖核苷酸合成的通用保护基团。
2-Diphenylmethylsilylethyl (DPSE): a versatile protecting group for oligodeoxyribonucleotide synthesis.
作者信息
Ravikumar V T, Cole D L
机构信息
Isis Pharmaceuticals, Carlsbad, CA 92008.
出版信息
Gene. 1994 Nov 4;149(1):157-61. doi: 10.1016/0378-1119(94)90424-3.
PMID:7958980
Abstract
2-Diphenylmethylsilylethyl (DPSE) is a new protecting group for the internucleotidic bonds in the solid-support and solution-phase synthesis of oligodeoxyribonucleotides by the phosphoramidite approach. This group is stable under acidic conditions and can be removed by a beta-fragmentation mechanism under mild conditions using aqueous NH4OH. Alternatively, this group can also be removed using tetrafluorosilane in acetonitrile.
摘要
2-二苯基甲基硅烷基乙基（DPSE）是一种用于通过亚磷酰胺方法在固相和溶液相中合成寡脱氧核糖核苷酸时保护核苷酸间键的新型保护基团。该基团在酸性条件下稳定，并且可以在温和条件下使用氨水通过β-断裂机制去除。或者，该基团也可以在乙腈中使用四氟硅烷去除。
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