从无杆蜥蜴卡罗来纳安乐蜥中克隆视紫红质编码基因。

Cloning of the rhodopsin-encoding gene from the rod-less lizard Anolis carolinensis.

作者信息

Kawamura S, Yokoyama S

机构信息

Department of Biology, Syracuse University, NY 13244.

出版信息

Gene. 1994 Nov 18;149(2):267-70. doi: 10.1016/0378-1119(94)90159-7.

DOI:10.1016/0378-1119(94)90159-7

PMID:7959000

Abstract

The rhodopsin (Rh) of the lizard Anolis carolinensis has only been detected in the pineal gland which controls circadian rhythms [Foster et al., J. Comp. Physiol. (1993) 33-45]. In the present study, we isolated and sequenced a genomic DNA clone for the A. carolinensis Rh gene. The deduced amino acid (aa) sequence revealed six A. carolinensis-specific replacements in conserved aa residues among vertebrate Rhs. Four out of these six replacements (S22N, N199H, E232A and T319M) reside outside the transmembrane (TM) domains. Some of these replacements might have been required for Rh to function solely in the pineal gland.

摘要

绿安乐蜥的视紫红质（Rh）仅在控制昼夜节律的松果体中被检测到[福斯特等人，《比较生理学杂志》（1993年）33 - 45页]。在本研究中，我们分离并测序了绿安乐蜥Rh基因的一个基因组DNA克隆。推导的氨基酸（aa）序列显示，在脊椎动物视紫红质保守的aa残基中有六个绿安乐蜥特有的替换。这六个替换中的四个（S22N、N199H、E232A和T319M）位于跨膜（TM）结构域之外。这些替换中的一些可能是视紫红质仅在松果体中发挥功能所必需的。