蜜蜂长波长视紫红质编码基因的克隆：一类新型昆虫视觉色素

Cloning of the gene encoding honeybee long-wavelength rhodopsin: a new class of insect visual pigments.

作者信息

Chang B S, Ayers D, Smith W C, Pierce N E

机构信息

Program in Neuroscience, Harvard University, Museum of Comparative Zoology Labs., Cambridge, MA 02138, USA.

出版信息

Gene. 1996 Sep 16;173(2):215-9. doi: 10.1016/0378-1119(96)00165-5.

DOI:10.1016/0378-1119(96)00165-5

PMID:8964502

Abstract

Rhodopsins (Rh), G-protein-coupled receptors with seven transmembrane (TM) helices, form the first step in visual transduction in most organisms. Although many long-wavelength (LW) vertebrate opsin sequences are known, less information is available for invertebrate LW sequences. By a combination of RT-PCR and cDNA library screening, we have cloned and sequenced the honeybee LW Rh gene. The deduced protein is composed of 378 amino acids (aa), appears to have seven TM regions, and contains many of the structures and key aa thought to be important for Rh function. Phylogenetic analysis of this sequence in relation to other invertebrate Rh reveals it to be a member of a new group of insect LW Rh.

摘要

视紫红质（Rh）是具有七个跨膜（TM）螺旋的G蛋白偶联受体，在大多数生物体的视觉转导中起第一步作用。尽管已知许多长波长（LW）脊椎动物视蛋白序列，但关于无脊椎动物LW序列的信息较少。通过逆转录聚合酶链反应（RT-PCR）和cDNA文库筛选相结合的方法，我们克隆并测序了蜜蜂LW Rh基因。推导的蛋白质由378个氨基酸（aa）组成，似乎有七个TM区域，并包含许多被认为对Rh功能很重要的结构和关键氨基酸。对该序列与其他无脊椎动物Rh进行系统发育分析，发现它是昆虫LW Rh新组的一个成员。