2”-O-糖基异荭草苷和α-生育酚对脂质过氧化系统中基因毒性乙二醛形成的抑制作用。

Inhibitory effect of 2"-O-glycosyl isovitexin and alpha-tocopherol on genotoxic glyoxal formation in a lipid peroxidation system.

作者信息

Nishiyama T, Hagiwara Y, Hagiwara H, Shibamoto T

机构信息

Department of Environmental Toxicology, University of California, Davis 95616.

出版信息

Food Chem Toxicol. 1994 Nov;32(11):1047-51. doi: 10.1016/0278-6915(94)90145-7.

DOI:10.1016/0278-6915(94)90145-7

PMID:7959459

Abstract

The inhibitory effect of 2"-O-glycosyl isovitexin (2"-O-GIV), isolated from young barley leaves, on glyoxal formation from the oxidative degradation of three fatty acid ethyl esters was measured. Ethyl linoleate, ethyl linolenate and ethyl arachidonate were oxidized by Fenton's reagent. Glyoxal formed from these fatty acid esters was analysed by gas chromatography after it had been derivatized to quinoxaline with 1,2-phenylenediamine. The comparative study was performed using alpha-tocopherol. Generally, alpha-tocopherol exhibited a greater inhibitory effect at lower levels, whereas 2"-O-GIV showed a greater effect than alpha-tocopherol at higher levels. 2"-O-GIV was more effective than alpha-tocopherol towards fatty acid esters with high numbers of double bonds. 2"-O-GIV exhibited a dose-response effect but alpha-tocopherol did not. Maximum inhibition of 82% was obtained from oxidation of ethyl arachidonate at 8 mumol 2"-O-GIV, whereas maximum inhibition of 77% was observed from oxidation of ethyl arachidonate at 0.25 mumol alpha-tocopherol.

摘要

测定了从嫩大麦叶中分离得到的2″-O-糖基异荭草素（2″-O-GIV）对三种脂肪酸乙酯氧化降解生成乙二醛的抑制作用。亚油酸乙酯、亚麻酸乙酯和花生四烯酸乙酯由芬顿试剂氧化。这些脂肪酸酯生成的乙二醛在用1,2-苯二胺衍生化为喹喔啉后，通过气相色谱法进行分析。使用α-生育酚进行了对比研究。一般来说，α-生育酚在较低水平时表现出更大的抑制作用，而2″-O-GIV在较高水平时比α-生育酚表现出更大的作用。2″-O-GIV对双键数量多的脂肪酸酯比α-生育酚更有效。2″-O-GIV呈现剂量反应效应，而α-生育酚则没有。在8 μmol 2″-O-GIV时，花生四烯酸乙酯氧化的最大抑制率为82%，而在0.25 μmol α-生育酚时，花生四烯酸乙酯氧化的最大抑制率为77%。